Recombinant Human ALAD Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ALAD-6108H
• Product Overview: ALAD MS Standard C13 and N15-labeled recombinant protein (NP_000022) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 37.23 kDa
• AA Sequence: MPLCPLAHAMQPQSVLHSGYFHPLL RAWQTATTTLNASNLIYPIFVTDVP DDIQPITSLPGVARYGVKRLEEMLR PLVEEGLRCVLIFGVPSRVPKDERG SAADSEESPAIEAIHLLRKTFPNLL VACDVCLCPYTSHGHCGLLSENGAF RAEESRQRLAEVALAYAKAGCQVVA PSDMMDGRVEAIKEALMAHGLGNRV SVMSYSAKFASCFYGPFRDAAKSSP AFGDRRCYQLPPGARGLALRAVDRD VREGADMLMVKPGMPYLDIVREVKD KHPDLPLAVYHVSGEFAMLWHGAQA GAFDLKAAVLEAMTAFRRAGADIII TYYTPQLLQWLKEESGPTRTRRLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ALAD aminolevulinate dehydratase [ Homo sapiens (human) ]
• Official Symbol: ALAD
• Synonyms: ALAD; aminolevulinate dehydratase; aminolevulinate, delta, dehydratase; delta-aminolevulinic acid dehydratase; ALADH; PBGS; porphobilinogen synthase; aminolevulinate, delta-, dehydratase; MGC5057
• Gene ID: 210
• mRNA Refseq: NM_000031
• Protein Refseq: NP_000022
• MIM: 125270
• UniProt ID: P13716