| Description : |
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Source : |
Insect cell |
| Species : |
Human |
| Molecular Mass : |
78 kDa |
| Protein length : |
1-674 |
| Purity : |
Clarified lysate |
| Unit Definition : |
One unit of enzyme consumes one nmol of oxygen per minute at 25 centigrade in 50 mM Tris-HCl buffer, pH7.5, containing 100μM arachidonate, 2 mM CaCl2, 1 mM ATP, 4.6 μM 13(S)-HpODE, and 250 μM oxygen. 5-Lipoxygenase activity is measured at 25 centigrade by monitoring oxygen consumption using a Gilson Model 5/6 H oxygraph equipped with a Clark oxygen electrode. |
| Stability : |
≥ 6 months |
| Storage : |
At -80 centigrade. |
| Storage Buffer : |
A clarified lysate, in 100 mM Tris-HCl, pH 8.0, containing 5 mM EGTA, 1mM CaCl2, and 30% glycerol |
