Recombinant Human ALPI protein, His-Flag-tagged

• Cat.No.: ALPI-4636H
• Product Overview: Recombinant Human ALPI protein(P09923)(Val20-Asp503), fused with N-terminal His-Flag tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: Val20-Asp503
• Tag: N-His-Flag
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Human Alkaline phosphatase (Intestinal type) , His Tag with an affinity constant of 1.58 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 54.5 kDa. Due to glycosylation, the protein migrates to 63-73 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ALPI alkaline phosphatase, intestinal [ Homo sapiens ]
• Official Symbol: ALPI
• Synonyms: ALPI; alkaline phosphatase, intestinal; intestinal-type alkaline phosphatase; Kasahara isozyme; glycerophosphatase; alkaline phosphomonoesterase; intestinal alkaline phosphatase; IAP
• Gene ID: 248
• mRNA Refseq: NM_001631
• Protein Refseq: NP_001622
• MIM: 171740
• UniProt ID: P09923