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Recombinant Human ALPP Protein, GST-tagged

Cat.No. : ALPP-495H
Product Overview : Human ALPP full-length ORF ( NP_001623.3, 1 a.a. - 535 a.a.) recombinant protein with GST-tag at N-terminal.
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  • Gene Information
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Description : The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 84.4 kDa
AA Sequence : MLGPCMLLLLLLLGLRLQLSLGIIP VEEENPDFWNREAAEALGAAKKLQP AQTAAKNLIIFLGDGMGVSTVTAAR ILKGQKKDKLGPEIPLAMDRFPYVA LSKTYNVDKHVPDSGATATAYLCGV KGNFQTIGLSAAARFNQCNTTRGNE VISVMNRAKKAGKSVGVVTTTRVQH ASPAGTYAHTVNRNWYSDADVPASA RQEGCQDIATQLISNMDIDVILGGG RKYMFRMGTPDPEYPDDYSQGGTRL DGKNLVQEWLAKRQGARYVWNRTEL MQASLDPSVTHLMGLFEPGDMKYEI HRDSTLDPSLMEMTEAALRLLSRNP RGFFLFVEGGRIDHGHHESRAYRAL TETIMFDDAIERAGQLTSEEDTLSL VTADHSHVFSFGGYPLRGSSIFGLA PGKARDRKAYTVLLYGNGPGYVLKD GARPDVTESESGSPEYRQQSAVPLD EETHAGEDVAVFARGPQAHLVHGVQ EQTFIAHVMAFAACLEPYTACDLAP PAGTTDAAHPGRSVVPALLPLLAGT LLLLETATAP
Applications : Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Gene Name : ALPP alkaline phosphatase, placental [ Homo sapiens ]
Official Symbol : ALPP
Synonyms : ALPP; alkaline phosphatase, placental; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; Regan isozyme; PLAP-1; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme; ALP; PALP; PLAP; FLJ61142;
Gene ID : 250
mRNA Refseq : NM_001632
Protein Refseq : NP_001623
MIM : 171800
UniProt ID : P05187

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can ALPP serve as a diagnostic biomarker or therapeutic target for specific diseases? 01/26/2022

ALPP may serve as a diagnostic biomarker for specific diseases, allowing for disease detection and monitoring, and its targeting could hold therapeutic potential in certain pathological conditions.

How does ALPP contribute to the metabolism and turnover of its substrate(s) in physiological and pathological conditions? 11/28/2021

ALPP participates in the metabolism and turnover of its substrate(s), including the hydrolysis of phosphoester bonds and the regulation of cellular phosphate homeostasis.

What are the downstream signaling pathways and molecular interactions regulated by ALPP in cellular functions? 12/22/2020

ALPP regulates downstream signaling pathways and molecular interactions involved in cellular processes like phosphate metabolism, intracellular signaling, and extracellular matrix remodeling.

What are the regulatory mechanisms controlling ALPP gene expression and enzyme activity? 12/15/2020

ALPP gene expression is regulated by transcription factors, epigenetic modifications, and signaling pathways involved in cellular homeostasis and disease processes.

What is the subcellular localization and trafficking of ALPP within cells? 11/09/2019

ALPP is localized to specific cellular compartments, such as the plasma membrane, endoplasmic reticulum, or lysosomes, and its trafficking may be regulated by sorting signals and intracellular transport machinery.

Can ALPP activity be modulated by specific factors or inhibitors, and how does this impact cellular processes? 07/17/2019

ALPP activity can be modulated by factors such as pH, calcium, and inhibitors, which can affect its catalytic efficiency and cellular functions.

How does ALPP deficiency or dysregulation contribute to the pathogenesis of diseases and disorders? 07/16/2019

ALPP deficiency or dysregulation is associated with various diseases, including metabolic disorders, bone diseases, and certain cancers, highlighting its pathological implications.

How can ALPP activity and function be manipulated to modulate cellular processes and disease outcomes? 09/12/2018

Manipulating ALPP activity and function could offer strategies to modulate cellular processes and disease outcomes, potentially leading to therapeutic interventions and improved patient outcomes.

How does ALPP expression and activity change during development, differentiation, and disease progression? 07/08/2018

ALPP expression and activity exhibit dynamic changes during development, cellular differentiation, and disease progression, reflecting its roles in different physiological and pathological contexts.

What is the expression pattern of ALPP in different tissues and cell types? 01/28/2017

ALPP shows a tissue-specific expression pattern, with higher levels in tissues such as liver, bone, and kidney, and lower levels in other organs.

Customer Reviews (3)

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Reviews
12/01/2020

    High-throughput screening compatibility.

    11/05/2018

      Consistent protein expression analysis.

      10/25/2017

        Precise protein quantification in ELISA.

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