Recombinant Human ANXA13 293 Cell Lysate

• Cat.No.: ANXA13-8835HCL

Specification

• Description: Antigen standard for annexin A13 (ANXA13), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: ANXA13 annexin A13 [ Homo sapiens ]
• Official Symbol: ANXA13
• Synonyms: ANXA13; annexin A13; ANX13; annexin-13; annexin XIII; intestine-specific annexin; annexin, intestine-specific; ISA; MGC150460
• Gene ID: 312
• mRNA Refseq: NM_001003954
• Protein Refseq: NP_001003954
• MIM: 602573
• UniProt ID: P27216
• Chromosome Location: 8q24.13
• Function: calcium ion binding; calcium-dependent phospholipid binding

Reviews

03/16/2021

Researchers can rely on the ANXA13 protein's specificity and sensitivity to obtain accurate and reliable results.

01/12/2020

the ANXA13 protein's versatility allows it to be effectively employed in various experimental designs, further enhancing its suitability for diverse research needs.

01/23/2019

The ANXA13 protein comes highly recommended for a variety of applications, particularly in ELISA and protein electron microscopy structure analysis.

11/19/2016

In protein electron microscopy structure analysis, the ANXA13 protein demonstrates outstanding performance.

Q&As

Are there any known mutations in the ANXA13 gene that are associated with disease?

Currently, there are no known mutations in the ANXA13 gene that have been directly linked to any specific diseases. However, genetic variations in the ANXA13 gene may exist and could potentially contribute to disease susceptibility or affect ANXA13 function. Further studies are needed to explore the genetic variability of ANXA13 and its potential implications in various diseases.

What cellular compartments or organelles is ANXA13 localized to?

ANXA13, like other annexin proteins, can be found in various cellular compartments or organelles. Annexin proteins have been detected on the plasma membrane, as well as in the cytoplasm, nucleus, mitochondria, endoplasmic reticulum, and lysosomes. The precise subcellular localization of ANXA13 may depend on the specific cell type and experimental conditions.

Are there any diseases associated with ANXA13 mutations or dysregulation?

Mutations in the ANXA13 gene have been associated with certain diseases, such as amyloidosis. Amyloidosis is a disorder characterized by the abnormal accumulation of proteins in various tissues and organs, leading to organ dysfunction. However, research into the specific role of ANXA13 mutations in the development of amyloidosis is still ongoing.

Are there any diseases associated with ANXA13 mutations or dysregulation?

Mutations in the ANXA13 gene have been associated with certain diseases, such as amyloidosis. Amyloidosis is a disorder characterized by the abnormal accumulation of proteins in various tissues and organs, leading to organ dysfunction. However, research into the specific role of ANXA13 mutations in the development of amyloidosis is still ongoing.

Is ANXA13 involved in cancer?

ANXA13 has been implicated in certain cancers. For example, studies have suggested that ANXA13 may promote gastric cancer progression by influencing tumor cell growth and metastasis. However, the exact mechanisms and extent of ANXA13 involvement in cancer development and progression are not fully understood and require further investigation.

Is ANXA13 involved in any physiological processes?

While the specific physiological processes involving ANXA13 are not well defined, annexins, in general, have been implicated in various cellular functions. Annexin proteins are known to be involved in membrane repair, membrane trafficking, regulation of ion channels, cell signaling, and apoptosis. It is possible that ANXA13 may have similar roles, but further research is needed to determine its specific functions.

In which tissues or organs is ANXA13 expressed?

ANXA13 is known to be expressed in various tissues, including the brain, kidney, liver, lung, and pancreas, among others. Its expression pattern may vary depending on the cell type and developmental stage.