Recombinant Human APP, His-GST tagged

• Cat.No.: APP-191H
• Product Overview: Recombinant Human APP (P05067-1) (Asp672-Gly709), fused with the N-terminal polyhistidine-tagged GST tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Fc
• Protein Length: 672-709 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 20% glycerol, 0.05% Tween 20, pH 9.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human Beta-APP38/GST chimera consists of 277 amino acids and has a calculated molecular mass of 32.1 KDa. It migrates as an approximately 34 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: APP amyloid beta (A4) precursor protein [ Homo sapiens ]
• Official Symbol: APP
• Gene ID: 351
• mRNA Refseq: NM_000484
• Protein Refseq: NP_000475
• MIM: 104760
• UniProt ID: P05067
• Chromosome Location: 21q21.2