Recombinant Human AR, Flag-tagged

• Cat.No.: AR-27312TH
• Product Overview: Recombinant human AR,fused with Flag tag, was expressed in Baculovirus-insect cell.

Specification

• Description: The AR, androgen receptor is an androgen-activated member of the nuclear receptor superfamily of transcription factors. Signaling by the androgen receptor plays a key role in proper development and function of male reproductive organs. The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. The androgen receptor (AR) has been shown to play a critical role in the development and progression of the prostate cancer.
• Source: Baculovirus-insect cell
• Species: Human
• Tag: Flag
• Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.
• Molecular Mass: 100.5 kDa
• AA Sequence: MEVQLGLGRV YPRPPSKTYR GAFQNLFQSV REVIQNPGPR HPEAASAAPP GASLLLLQQQ QQQQQQQQQQ QQQQQQQQQQ ETSPRQQQQQ QGEDGSPQAH RRGPTGYLVL DEEQQPSQPQSALECHPERG CVPEPGAAVA ASKGLPQQLP APPDEDDSAA PSTLSLLGPT FPGLSSCSAD LKDILSEAST MQLLQQQQQE AVSEGSSSGR AREASGAPTS SKDNYLGGTS TISDNAKELCKAVSVSMGLG VEALEHLSPG EQLRGDCMYA PLLGVPPAVR PTPCAPLAEC KGSLLDDSAG KSTEDTAEYS PFKGGYTKGL EGESLGCSGS AAAGSSGTLE LPSTLSLYKS GALDEAAAYQSRDYYNFPLA LAGPPPPPPP PHPHARIKLE NPLDYGSAWA AAAAQCRYGD LASLHGAGAA GPGSGSPSAA ASSSWHTLFT AEEGQLYGPC GGGGGGGGGG GGGGGGGGGG GGGEAGAVAPYGYTRPPQGL AGQESDFTAP DVWYPGGMVS RVPYPSPTCV KSEMGPWMDS YSGPYGDMRL ETARDHVLPI DYYFPPQKTC LICGDEASGC HYGALTCGSC KVFFKRAAEG KQKYLCASRNDCTIDKFRRK NCPSCRLRKC YEAGMTLGAR KLKKLGNLKL QEEGEASSTT SPTEETTQKL TVSHIEGYEC QPIFLNVLEA IEPGVVCAGH DNNQPDSFAA LLSSLNELGE RQLVHVVKWAKALPGFRNLH VDDQMAVIQY SWMGLMVFAM GWRSFTNVNS RMLYFAPDLV FNEYRMHKSR MYSQCVRMRH LSQEFGWLQI TPQEFLCMKA LLLFSIIPVD GLKNQKFFDE LRMNYIKELDRIIACKRKNP TSCSRRFYQL TKLLDSVQPI ARELHQFTFD LLIKSHMVSV DFPEMMAEII SVQVPKILSG KVKPIYFHTQ
• Purity: The Flag-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified Androgen Receptor is greater than 90% homogeneous based on SDS-PAGE analysis.
• Unit Definition: 1 unit equals 1 nanogram of purified protein.
• Applications: For Research Use only and not for Drug or Diagnostic purposes. AR can be used for gel mobility shift assay, for protein-protein and small molecules-protein interactions assay.
• Publication:
  A detailed characterization of stepwise activation of the androgen receptor variant 7 in prostate cancer cells (2020)
  Therapeutic targeting of BET bromodomain proteins in castration-resistant prostate cancer (2014)

Gene Information

• Gene Name: AR androgen receptor [ Homo sapiens ]
• Official Symbol: AR
• Synonyms: KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; androgen receptor; dihydrotestosterone receptor; androgen nuclear receptor variant 2; nuclear receptor subfamily 3 group C member 4
• Gene ID: 367
• mRNA Refseq: NM_000044
• Protein Refseq: NP_000035
• MIM: 313700
• UniProt ID: P10275
• Chromosome Location: Xq12
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen receptor signaling pathway, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem
• Function: DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; positive regulation of transcription