Recombinant Human ARNT, GST-tagged

• Cat.No.: ARNT-20H
• Product Overview: Recombinant Human ARNT(1 a.a. - 789 a.a.), fused with GST-tag at N-terminal, was expressed in wheat germ.

Specification

• Description: This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants.
• Source: Wheat germ
• Species: Human
• Tag: GST
• Molecular Mass: 113 kDa
• AA Sequence: MAATTANPEMTSDVPSLGPAIASGN SGPGIQGGGAIVQRAIKRRPGLDFD DDGEGNSKFLRCDDDQMSNDKERFA RSDDEQSSADKERLARENHSEIERR RRNKMTAYITELSDMVPTCSALARK PDKLTILRMAVSHMKSLRGTGNTST DGSYKPSFLTDQELKHLILEAADGF LFIVSCETGRVVYVSDSVTPVLNQP QSEWFGSTLYDQVHPDDVDKLREQL STSENALTGRILDLKTGTVKKEGQQ SSMRMCMGSRRSFICRMRCGSSSVD PVSVNRLSFVRNRCRNGLGSVKDGE PHFVVVHCTGYIKAWPPAGVSLPDD DPEAGQGSKFCLVAIGRLQVTSSPN CTDMSNVCQPTEFISRHNIEGIFTF VDHRCVATVGYQPQELLGKNIVEFC HPEDQQLLRDSFQQVVKLKGQVLSV MFRFRSKNQEWLWMRTSSFTFQNPY SDEIEYIICTNTNVKNSSQEPRPTL SNTIQRPQLGPTANLPLEMGSGQLA PRQQQQQTELDMVPGRDGLASYNHS QVVQPVTTTGPEHSKPLEKSDGLFA QDRDPRFSEIYHNINADQSKGISSS TVPATQQLFSQGNTFPPTPRPAENF RNSGLAPPVTIVQPSASAGQMLAQI SRHSNPTQGATPTWTPTTRSGFSAQ QVATQATAKTRTSQFGVGSFQTPSS FSSMSLPGAPTASPGAAAYPSLTNR GSNFAPETGQTAGQFQTRTAEGVGV WPQWQGQQPHHRSSSSEQHVQQPPA QQPGQPEVFQEMLSMLGDQSNSYNN EEFPDLTMFPPFSE
• Applications: ELISA; WB-Re; AP; Array
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Publication:
  Hypoxia disrupts aryl hydrocarbon receptor signaling and the Th17 response in allergic rhinitis patients (2018)

Gene Information

• Gene Name: ARNT aryl hydrocarbon receptor nuclear translocator [ Homo sapiens (human) ]
• Official Symbol: ARNT
• Synonyms: ARNT; aryl hydrocarbon receptor nuclear translocator; bHLHe2; HIF 1beta; dioxin receptor, nuclear translocator; class E basic helix-loop-helix protein 2; hypoxia-inducible factor 1, beta subunit; HIF1B; TANGO; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
• Gene ID: 405
• mRNA Refseq: NM_001668
• Protein Refseq: NP_001659
• MIM: 126110
• UniProt ID: P27540
• Chromosome Location: 1q21
• Pathway: AhR pathway; Cellular response to hypoxia; Chemical carcinogenesis
• Function: contributes_to RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; aryl hydrocarbon receptor activity; aryl hydrocarbon receptor binding