Recombinant Full Length Human ARNT, Flag-tagged

• Cat.No.: ARNT-1433HFL
• Product Overview: Recombinant Full Length Human ARNT, fused with C-terminal Flag tag, was expressed in mamanlian cells.

Specification

• Source: Mamanlian cells
• Species: Human
• Tag: Flag
• Molecular Mass: 86.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: ARNT aryl hydrocarbon receptor nuclear translocator [ Homo sapiens (human) ]
• Official Symbol: ARNT
• Synonyms: ARNT; aryl hydrocarbon receptor nuclear translocator; bHLHe2; HIF 1beta; dioxin receptor, nuclear translocator; class E basic helix-loop-helix protein 2; hypoxia-inducible factor 1, beta subunit; HIF1B; TANGO; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
• Gene ID: 405
• mRNA Refseq: NM_001668
• Protein Refseq: NP_001659
• MIM: 126110
• UniProt ID: P27540

Reviews

07/03/2022

This protein demonstrates great performance in WB assays, providing accurate and reliable results that are crucial for protein analysis.

11/16/2021

Incorporating this protein into scientific investigations undoubtedly enhances the reliability and effectiveness of research endeavors.

06/17/2019

The ARNT protein is highly recommended for researchers conducting protein studies, especially in Western blotting (WB) experiments.

02/04/2019

the ARNT protein has proven to be an invaluable tool in protein electron microscopy (EM) structure analysis.

10/26/2018

Whether investigating metabolic pathways, studying enzymatic activity, or exploring drug targets, this protein offers versatility and reliability, allowing for accurate and meaningful data collection.

07/24/2018

This level of support instills trust and confidence in the product, facilitating smooth and successful experimentation.

Q&As

Is the ARNT protein primarily localized in the nucleus?

The ARNT protein primarily localizes to the nucleus, where it functions as a transcription factor. However, under certain conditions, it can shuttle between the nucleus and cytoplasm, depending on the specific cellular signals and protein interactions involved.

Can the ARNT protein interact with other proteins?

Yes, the ARNT protein can interact with various proteins, both within the cytoplasm and the nucleus. It forms heterodimers with partners like AhR, hypoxia-inducible factor (HIF), and hypoxia-responsive transcription factors, among others, to regulate gene expression.

How does the ARNT protein contribute to hypoxia adaptation?

The ARNT protein is a key player in the cellular response to hypoxia (low oxygen levels). When oxygen levels are low, ARNT forms a complex with HIF-1α or HIF-2α, leading to the expression of genes involved in angiogenesis, erythropoiesis, and glucose metabolism, helping cells adapt and survive in oxygen-deprived environments.

What are the structural features of the ARNT protein?

The ARNT protein belongs to the basic helix-loop-helix (bHLH) family of transcription factors. It contains multiple functional domains, including the bHLH domain, Per-ARNT-Sim (PAS) domains, and a transactivation domain for interacting with other transcriptional co-regulators.

Is ARNT expression altered in different tissues or cell types?

Yes, the expression of ARNT can vary in different tissues and cell types. This can be due to tissue-specific transcriptional regulation, epigenetic modifications, and cell-specific signaling pathways. The expression and activity of ARNT can also be influenced by developmental stages and environmental factors.

Can ARNT play a role in immune response regulation?

Yes, the ARNT protein can play a role in immune response regulation. It has been shown to be involved in the differentiation and function of immune cells, such as T cells and natural killer cells. ARNT can regulate the expression of genes involved in immune response pathways, including cytokine production and immune cell activation.

Can ARNT regulate the expression of its own gene?

Yes, ARNT can regulate the expression of its own gene through a positive feedback loop. For example, the ARNT protein can bind to specific regulatory sequences in the ARNT gene promoter and enhance its own transcription, leading to increased ARNT expression.

Can ARNT be part of protein complexes other than those involving transcription factors?

Yes, ARNT can participate in protein complexes other than those involving transcription factors. For example, it can form complexes with co-activators or co-repressors, chromatin remodeling proteins, and other regulatory proteins involved in transcriptional regulation or protein stability.

Does the ARNT protein have any role in drug metabolism?

Yes, the ARNT protein is involved in the regulation of drug metabolism by modulating the expression of phase I and phase II drug-metabolizing enzymes and transporters.

What are some known transcription factors that interact with the ARNT protein?

Some known transcription factors that interact with the ARNT protein include aryl hydrocarbon receptor (AhR), hypoxia-inducible factor (HIF), hypoxia-responsive transcription factors (HRTFs), and estrogen receptor alpha (ERα).

Are there any drugs that target the ARNT protein?

Currently, there are no specific drugs available that directly target the ARNT protein. However, research is ongoing to identify compounds and strategies that can modulate ARNT activity and downstream gene expression for potential therapeutic purposes.

What are the physiological processes influenced by the ARNT protein?

The ARNT protein plays a crucial role in diverse physiological processes. It has been implicated in the regulation of xenobiotic metabolism, response to environmental toxins, development of vascular and respiratory systems, immune response, and adaptation to hypoxia.

Are there any diseases associated with dysfunctional ARNT protein?

Dysregulation or mutation of the ARNT protein has been linked to several diseases, including various cancers, metabolic disorders, and developmental abnormalities.

Are there any diseases or conditions associated with dysregulation of ARNT?

Dysregulation of ARNT has been implicated in various diseases and conditions. For example, alterations in ARNT expression or activity can contribute to metabolic disorders like obesity and diabetes. Dysregulated ARNT signaling has also been linked to certain cancers, cardiovascular diseases, and neurodegenerative disorders.

Can ARNT be regulated by small molecules or environmental factors?

Yes, ARNT can be regulated by small molecules and environmental factors. For example, the activity of ARNT can be modulated by ligands that bind to its interacting partner, such as the aryl hydrocarbon receptor (AhR). Environmental pollutants, such as dioxins and polycyclic aromatic hydrocarbons (PAHs), can bind to AhR and indirectly affect ARNT activity.

Can the ARNT protein play a role in cancer development?

Yes, dysregulation of the ARNT protein has been implicated in various cancers. It can influence tumor growth, angiogenesis, and drug resistance through its interactions with other transcription factors, such as HIF-1α and AhR, and the modulation of downstream target genes involved in these processes.

Can the ARNT protein be modified by phosphorylation?

Yes, the ARNT protein can undergo phosphorylation, which can impact its stability, activity, and interactions with other proteins. Phosphorylation events influenced by various kinases can regulate the function of ARNT in different cellular contexts.

What are some additional roles of the ARNT protein in development?

Besides its involvement in the development of the vascular and respiratory systems, the ARNT protein also plays a role in embryonic development, including organogenesis, neural development, and placental development. It regulates the expression of genes essential for proper development during these processes.

Does ARNT have any role in oxidative stress response?

Yes, ARNT plays a role in oxidative stress response. It can regulate the expression of genes involved in antioxidant defense mechanisms, such as the expression of enzymes involved in detoxification processes and protection against reactive oxygen species (ROS).

Can ARNT interact with other proteins or factors not mentioned earlier?

Yes, in addition to AhR and HIF, ARNT can interact with a wide range of other proteins and transcription factors. Some examples include p53, NF-κB, c-Myc, STAT3, and Nrf2. These interactions contribute to the diverse functions of ARNT in different cellular processes and signaling pathways.

Can the ARNT protein be targeted for therapeutic interventions?

Given its involvement in various diseases and its role in regulating gene expression, the ARNT protein is an attractive target for therapeutic interventions. However, more research is needed to explore specific strategies and compounds that can modulate its activity.

Are there any inherited disorders associated with ARNT protein mutations?

Yes, mutations in the ARNT gene have been associated with a few inherited disorders. For example, some individuals with a rare genetic disorder called aryl hydrocarbon receptor deficiency (AHRD) have mutations in the ARNT gene, leading to impaired AhR signaling and dysfunctional immune response.

Can the ARNT protein influence cellular metabolism?

Yes, the ARNT protein plays a role in cellular metabolism by regulating the expression of genes involved in xenobiotic metabolism, energy metabolism, and lipid metabolism. It can impact the activity of drug-metabolizing enzymes and certain metabolic pathways under various environmental and physiological conditions.