Recombinant Human ARPM1 protein, His-tagged

• Cat.No.: ARPM1-3949H
• Product Overview: Recombinant Human ARPM1 protein(16-233 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 16-233 aa
• AA Sequence: MIKAGVAGCREPQFIYPNIIGRAKG QSRAAQGGLELCVGDQAQDWRSSLF ISYPVERGLITSCEDMEIMWKHIYD YNLKLKPCDGPVLITEPALNPLANR QQITEMFFEHLGVPAFYMSIQAVLA LFAAGFTTGLVLNSGAGVTQSVPIF EGYCLPHGVQQLDLAGLDLTNYLMV LMKNHGIMLLSASDRKIVEDIKESF CYVAMNYEEEMAKKPDCL
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Reviews

11/20/2022

The ARPM1 protein stands out as a protein of exceptional quality, perfectly suited to meet the experimental needs of researchers.

04/21/2020

Whether utilized in protein-protein interaction studies or enzymatic assays, this protein exhibits robust performance, enabling accurate data generation and analysis.

09/01/2017

With its reliable performance and consistent results, the ARPM1 protein provides researchers with the confidence and accuracy required for successful experimentation.

Q&As

Is ARPM1 protein regulated by any post-translational modifications?

The specific post-translational modifications of ARPM1 are not well-studied. However, like other proteins in the Arp2/3 complex, it is likely that ARPM1 can undergo modifications such as phosphorylation, acetylation, or ubiquitination to regulate its function.

Does ARPM1 protein have any unique structural features?

ARPM1 protein contains structural domains similar to other subunits of the Arp2/3 complex. It has an alpha-helical domain that interacts with other subunits and an extended ARM/HEAT repeat domain involved in protein-protein interactions and actin binding.

Can ARPM1 protein be used as a diagnostic or prognostic marker for any diseases?

At present, ARPM1 protein has not been established as a clinically useful diagnostic or prognostic marker for any specific diseases. However, further research might uncover its potential utility in disease diagnosis or prognosis.

Is ARPM1 protein involved in any diseases?

Limited studies have linked dysregulation of the Arp2/3 complex, including ARPM1, with certain diseases such as cancer, neurological disorders, and autoimmune diseases. However, more research is needed to understand its specific roles in disease progression.

Are there any known genetic variations or mutations in the ARPM1 gene?

While specific variations or mutations in the ARPM1 gene have not been extensively studied, genetic variations in other subunits of the Arp2/3 complex have been associated with diseases. It's possible that variations in the ARPM1 gene may have functional implications, but further research is needed.

Are there any known cellular processes that require ARPM1 protein?

ARPM1 protein is crucial for various cellular processes where actin dynamics play a significant role, including cell migration, cell shape changes, cell adhesion, endocytosis, vesicle trafficking, and membrane remodeling.

Can ARPM1 protein interact with other proteins or complexes?

ARPM1 interacts with other subunits of the Arp2/3 complex, as well as actin-binding proteins, signaling molecules, and adaptor proteins. These interactions are essential for the proper functioning of the Arp2/3 complex and actin dynamics.