Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human ASAH2 Cell Lysate, Myc/DDK-tagged

Cat.No. : ASAH2-365HCL
Product Overview : Transient overexpression lysate of N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2), transcript variant 1 with a C-Myc/DDK tag was expressed in HEK293T.
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5 μg TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25 mM Tris-HCl pH7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20 centigrade before shipping.
Components:
1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 μg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250 μL 2×SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100 mM DTT)
  • Specification
  • Gene Information
  • Related Products
Description : Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.
Source : HEK293T
Species : Human
Tag : Myc/DDK
Molecular Mass : 83.1 kDa
Stability : Avoid repeated freeze-thaw cycles.
Storage : Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade.
Reconstitution : Lysate samples can be diluted with 2×SDS Sample Buffer provided.
Shipping : Dry ice
Gene Name : ASAH2 N-acylsphingosine amidohydrolase 2 [ Homo sapiens (human) ]
Official Symbol : ASAH2 N-acylsphingosine amidohydrolase 2 [ Homo sapiens (human) ]
Synonyms : ASAH2; N-acylsphingosine amidohydrolase 2; HNAC1; BCDase; LCDase; NCDase; N-CDase; neutral ceramidase; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; acylsphingosine deacylase 2; mitochondrial ceramidase; neutral/alkaline ceramidase; non-lysosomal ceramidase; EC 3.5.1.23
Gene ID : 56624
mRNA Refseq : NM_019893
Protein Refseq : NP_063946
MIM : 611202
UniProt ID : Q9NR71

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
Can ASAH2 activity be regulated by post-translational modifications? 01/27/2022

Post-translational modifications, such as phosphorylation, may impact ASAH2 activity, although further research is needed for a comprehensive understanding.

Are there any known inhibitors of ASAH2? 08/27/2019

While specific inhibitors for ASAH2 are not yet available, researchers are actively exploring potential compounds that can modulate its activity.

In which cellular compartments is ASAH2 found? 01/28/2018

ASAH2 is primarily localized in the lysosomes, where it carries out the ceramide hydrolysis process.

What are the consequences of ASAH2 deficiency? 10/09/2017

Deficiency in ASAH2 activity can lead to the accumulation of ceramides, which may disrupt cellular signaling and contribute to disease development.

How is ASAH2 gene expression regulated? 01/28/2017

ASAH2 gene expression can be regulated by various factors, including transcription factors, epigenetic modifications, and signaling pathways.

Are there any diseases associated with ASAH2? 09/17/2016

Mutations in the ASAH2 gene have been associated with a rare form of hereditary sensory and autonomic neuropathy (HSAN) called HSAN1C.

Is ASAH2 ubiquitously expressed in all tissues? 08/08/2016

Yes, ASAH2 is ubiquitously expressed in various tissues throughout the body.

Customer Reviews (3)

Write a review
Reviews
04/04/2019

    It reliably and accurately detects and quantifies target molecules, allowing for precise measurement of analytes in my samples.

    02/25/2019

      I highly recommend the ASAH2 Protein for its outstanding performance in ELISA assays.

      11/22/2016

        The sensitivity and specificity of the ASAH2 Protein have consistently delivered robust and consistent results, enhancing the efficiency and accuracy of my experimental workflows.

        Ask a Question for All ASAH2 Products

        Required fields are marked with *

        My Review for All ASAH2 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends