Recombinant Human ATG10 Autophagy Related 10 Homolog (S. cerevisiae), His-tagged

• Cat.No.: ATG10-7174H
• Product Overview: Recombinant human ATG10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 27.7 kDa.

Specification

• Description: Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT.
• Molecular Mass: 27.7 kDa (243aa) confirmed by MALDI-TOF
• Protein length: 1-220aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ATG10 ATG10 autophagy related 10 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: ATG10
• Synonyms: ATG10; ATG10 autophagy related 10 homolog (S. cerevisiae); APG10 autophagy 10 like (S. cerevisiae) , APG10L; ubiquitin-like-conjugating enzyme ATG10; DKFZP586I0418; FLJ13954; autophagy-related protein 10; APG10; APG10L; pp12616; DKFZp586I0418
• Gene ID: 83734
• mRNA Refseq: NM_001131028
• Protein Refseq: NP_001124500
• MIM: 610800
• UniProt ID: Q9H0Y0
• Chromosome Location: 5q14.1
• Pathway: Senescence and Autophagy, organism-specific biosystem
• Function: Atg12 ligase activity; ligase activity