Recombinant Human ATP6V0A1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human ATP6V0A1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : ATP6V0A1-2980H
Product Overview : ATP6V0A1 MS Standard C13 and N15-labeled recombinant protein (NP_005168) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 95.6 kDa
AA Sequence : MGELFRSEEM TLAQLFLQSE AAYCCVSELG ELGKVQFRDL NPDVNVFQRK FVNEVRRCEE MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN FEKIENELKE INTNQEALKR NFLELTELKF ILRKTQQFFD EMADPDLLEE SSSLLEPSEM GRGTPLRLGF VAGVINRERI PTFERMLWRV CRGNVFLRQA EIENPLEDPV TGDYVHKSVF IIFFQGDQLK NRVKKICEGF RASLYPCPET PQERKEMASG VNTRIDDLQM VLNQTEDHRQ RVLQAAAKNI RVWFIKVRKM KAIYHTLNLC NIDVTQKCLI AEVWCPVTDL DSIQFALRRG TEHSGSTVPS ILNRMQTNQT PPTYNKTNKF TYGFQNIVDA YGIGTYREIN PAPYTIITFP FLFAVMFGDF GHGILMTLFA VWMVLRESRI LSQKNENEMF STVFSGRYII LLMGVFSMYT GLIYNDCFSK SLNIFGSSWS VRPMFTYNWT EETLRGNPVL QLNPALPGVF GGPYPFGIDP IWNIATNKLT FLNSFKMKMS VILGIIHMLF GVSLSLFNHI YFKKPLNIYF GFIPEIIFMT SLFGYLVILI FYKWTAYDAH TSENAPSLLI HFINMFLFSY PESGYSMLYS GQKGIQCFLV VVALLCVPWM LLFKPLVLRR QYLRRKHLGT LNFGGIRVGN GPTEEDAEII QHDQLSTHSE DADEFDFGDT MVHQAIHTIE YCLGCISNTA SYLRLWALSL AHAQLSEVLW TMVIHIGLSV KSLAGGLVLF FFFTAFATLT VAILLIMEGL SAFLHALRLH WVEFQNKFYS GTGFKFLPFS FEHIREGKFE ETRTRPLEQK LISEEDLAAN DILDYKDDDD KV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : ATP6V0A1 ATPase H+ transporting V0 subunit a1 [ Homo sapiens (human) ]
Official Symbol : ATP6V0A1
Synonyms : ATP6V0A1; ATPase, H+ transporting, lysosomal V0 subunit a1; ATP6N1, ATP6N1A, ATPase, H+ transporting, lysosomal (vacuolar proton pump) non catalytic accessory protein 1A (110/116kD), ATPase, H+ transporting, lysosomal V0 subunit a isoform 1, ATPase, H+ transporting, lysosomal V0 subunit A1, VPP1; V-type proton ATPase 116 kDa subunit a isoform 1; a1; Stv1; Vph1; V-ATPase 116 kDa; vacuolar proton pump subunit 1; vacuolar proton pump, subunit 1; V-type proton ATPase 116 kDa subunit a; vacuolar-type H(+)-ATPase 115 kDa subunit; vacuolar adenosine triphosphatase subunit Ac116; vacuolar proton translocating ATPase 116 kDa subunit A; H(+)-transporting two-sector ATPase, 116 kDa accessory protein A1; clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit; ATPase, H+ transporting, lysosomal non-catalytic accessory protein 1 (110/116kD); ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1A (110/116kD); VPP1; ATP6N1; ATP6N1A; DKFZp781J1951;
Gene ID : 535
mRNA Refseq : NM_005177
Protein Refseq : NP_005168
MIM : 192130
UniProt ID : Q93050
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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