Recombinant Human ATP6V1B2 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human ATP6V1B2 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : ATP6V1B2-2300H
Product Overview : ATP6V1B2 MS Standard C13 and N15-labeled recombinant protein (NP_001684) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 56.4 kDa
AA Sequence : MALRAMRGIV NGAAPELPVP TGGPAVGAQE QALAVSRNYL SQPRLTYKTV SGVNGPLVIL DHVKFPRYAE IVHLTLPDGT KRSGQVLEVS GSKAVVQVFE GTSGIDAKKT SCEFTGDILR TPVSEDMLGR VFNGSGKPID RGPVVLAEDF LDIMGQPINP QCRIYPEEMI RTGISAIDGM NSIARGQKIP IFSAAGLPHN EIAAQICRQA GLVKKSKDVV DYSEENFAIV FAAMGVNMET ARFFKSDFEE NGSMDNVCLF LNLANDPTIE RIITPRLALT TAEFLAYQCE KHVLVILTDM SSYAEALREV SAAREEVPGR RGFPGYMYTD LATIYERAGR VGGRNGSITQ IPILTMPNDD ITHPIPDLTG YITEGQIYVD RQLHNRQIYP PINVLPSLSR LMKSAIGEGM TRKDHADVSN QLYACYAIGK DVQAMKAVVG EEALTSDDLL YLEFLQKFER NFIAQGPYEN RTVFETLDIG WQLLRIFPKE MLKRIPQSTL SEFYPRDSAK HTRTRPLEQK LISEEDLAAN DILDYKDDDD KV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : ATP6V1B2 ATPase H+ transporting V1 subunit B2 [ Homo sapiens (human) ]
Official Symbol : ATP6V1B2
Synonyms : ATP6V1B2; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2; ATP6B2, ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide, 56/58kD, isoform 2, ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2, VPP3; V-type proton ATPase subunit B, brain isoform; HO57; VATB; Vma2; V-ATPase B2 subunit; V-ATPase subunit B 2; vacuolar proton pump subunit B 2; H+ transporting two-sector ATPase; vacuolar H+-ATPase 56,000 subunit; endomembrane proton pump 58 kDa subunit; VPP3; ATP6B2; ATP6B1B2;
Gene ID : 526
mRNA Refseq : NM_001693
Protein Refseq : NP_001684
MIM : 606939
UniProt ID : P21281

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