Description : |
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. |
Source : |
E. Coli |
Species : |
Human |
Tag : |
His |
Form : |
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
Molecular Mass : |
32.7kDa |
Protein length : |
843 |
Endotoxin : |
<1.0eu per 1ug (determined by the lal</1.0eu |
Purity : |
>95% |
Applications : |
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) |
Notes : |
Small volumes of AXIN2 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition. |
Reconstitution : |
Reconstitute in sterile PBS, pH7.2-pH7.4. |
Warning : |
This product is for research use only. Not for use in diagnostic or therapeutic procedures. |