Recombinant Human BAG3, His-tagged

• Cat.No.: BAG3-10125H
• Product Overview: Recombinant Human BAG3 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 275-575a.a.
• Description: BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: BAG3 BCL2-associated athanogene 3 [ Homo sapiens ]
• Official Symbol: BAG3
• Synonyms: BAG3; BCL2-associated athanogene 3; BAG family molecular chaperone regulator 3; docking protein CAIR-1; BCL2-binding athanogene 3; bcl-2-binding protein Bis; bcl-2-associated athanogene 3; BAG-family molecular chaperone regulator-3; BIS; BAG-3; CAIR-1; MGC104307
• Gene ID: 9531
• mRNA Refseq: NM_004281
• Protein Refseq: NP_004272
• MIM: 603883
• UniProt ID: O95817
• Chromosome Location: 10q25.2-q26.2
• Function: chaperone binding