Description : |
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
61.4 kDa |
AA Sequence : |
MSAATHSPMMQVASGNGDRDPLPPG WEIKIDPQTGWPFFVDHNSRTTTWN DPRVPSEGPKETPSSANGPSREGSR LPPAREGHPVYPQLRPGYIPIPVLH EGAENRQVHPFHVYPQPGMQRFRTE AAAAAPQRSQSPLRGMPETTQPDKQ CGQVAAAAAAQPPASHGPERSQSPA ASDCSSSSSSASLPSSGRSSLGSHQ LPRGYISIPVIHEQNVTRPAAQPSF HQAQKTHYPAQQGEYQTHQPVYHKI QGDDWEPRPLRAASPFRSSVQGASS REGSPARSSTPLHSPSPIRVHTVVD RPQQPMTHRETAPVSQPENKPESKP GPVGPELPPGHIPIQVIRKEVDSKP VSQKPPPPSEKVEVKVPPAPVPCPP PSPGPSAVPSSPKSVATEERAAPST APAEATPPKPGEAEAPPKHPGVLKV EAILEKVQGLEQAVDNFEGKKTDKK YLMIEEYLTKELLALDSVDPEGRAD VRQARRDGVRKVQTILEKLEQKAID VPGQVQVYELQPSNLEADQPLQAIM EMGAVAADKGKKNAGNAEDPHTETQ QPEATAAATSNPSSMTDTPGNPAAP TRTRPLEQKLISEEDLAANDILDYK DDDDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |