Recombinant Human BCAT1 Protein, Myc/DDK-tagged, C13 and N15-labeled
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| Cat.No. : | BCAT1-1842H |
| Product Overview : | BCAT1 MS Standard C13 and N15-labeled recombinant protein (NP_005495) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells. |
| Description : | This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. |
| Source : | HEK293 |
| Species : | Human |
| Tag : | Myc/DDK |
| Molecular Mass : | 42.8 kDa |
| AA Sequence : | MKDCSNGCSA ECTGEGGSKE VVGTFKAKDL IVTPATILKE KPDPNNLVFG TVFTDHMLTV EWSSEFGWEK PHIKPLQNLS LHPGSSALHY AVELFEGLKA FRGVDNKIRL FQPNLNMDRM YRSAVRATLP VFDKEELLEC IQQLVKLDQE WVPYSTSASL YIRPTFIGTE PSLGVKKPTK ALLFVLLSPV GPYFSSGTFN PVSLWANPKY VRAWKGGTGD CKMGGNYGSS LFAQCEAVDN GCQQVLWLYG EDHQITEVGT MNLFLYWINE DGEEELATPP LDGIILPGVT RRCILDLAHQ WGEFKVSERY LTMDDLTTAL EGNRVREMFG SGTACVVCPV SDILYKGETI HIPTMENGPK LASRILSKLT DIQYGREESD WTIVLSTRTR PLEQKLISEE DLAANDILDY KDDDDKV |
| Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : | Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : | Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : | 50 μg/mL as determined by BCA |
| Storage Buffer : | 100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
| SDS-PAGE : |
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| Gene Name : | BCAT1 branched chain amino acid transaminase 1 [ Homo sapiens (human) ] |
| Official Symbol : | BCAT1 |
| Synonyms : | BCAT1; branched chain amino-acid transaminase 1, cytosolic; BCT1, branched chain aminotransferase 1, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; placental protein 18; branched chain aminotransferase 1, cytosolic; BCT1; PP18; BCATC; ECA39; MECA39; PNAS121; DKFZp686E12175; |
| Gene ID : | 586 |
| mRNA Refseq : | NM_005504 |
| Protein Refseq : | NP_005495 |
| MIM : | 113520 |
| UniProt ID : | P54687 |
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◆ Lysates
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