Recombinant Human BMP2

• Cat.No.: BMP2-26315TH
• Product Overview: Recombinant Human BMP2. A 26 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 283-396 aa
• Description: The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation.
• Tissue specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
• Form: Lyophilised:Centrifuge the vial prior to opening.Reconstitute in water containing BSA (50ug of BSA per 1ug of protein) to a concentration of 0.1-1.0 mg/ml.This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C f
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneEndotoxin level is less than 0.1 ng per μg (1EU/μg).
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Sequences of amino acids: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
• Sequence Similarities: Belongs to the TGF-beta family.

Gene Information

• Gene Name: BMP2 bone morphogenetic protein 2 [ Homo sapiens ]
• Official Symbol: BMP2
• Synonyms: BMP2; bone morphogenetic protein 2; BMP2A
• Gene ID: 650
• mRNA Refseq: NM_001200
• Protein Refseq: NP_001191
• MIM: 112261
• Uniprot ID: P12643
• Chromosome Location: 20p12
• Pathway: Adipogenesis, organism-specific biosystem; Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem
• Function: BMP receptor binding; SMAD binding; activin receptor activity, type II; cytokine activity; growth factor activity

Citation

Controlled release of injectable liposomal in situ gel loaded with recombinant human bone morphogenetic protein-2 for the repair of alveolar bone clefts in rabbits.

Journal: Journal of liposome research    PubMed ID: 26152279    Data: 2017/1/9

Authors: Ali H Hassan, Khaled M Hosny, Ahmed R Afify

Article Snippet:rhBMP-2 was purchased from Creative BioMart (New York, NY).. Phosphatidylcholine (extracted from chicken egg yolk), lecithin, cholesterol and DGG were purchased from Sigma Chemical Company (St. Louis, MO).Phosphatidylcholine (extracted from chicken egg yolk), lecithin, cholesterol and DGG were purchased from Sigma Chemical Company (St. Louis, MO).

Depot injectable biodegradable nanoparticles loaded with recombinant human bone morphogenetic protein-2: preparation, characterization, and in vivo evaluation

Journal: Drug Design, Development and Therapy    Data: 2015/7/1

Authors: Ali Hassan, Khaled Hosny, Ghada Naguib

Article Snippet:Materials rhBMP-2 was purchased from Creative BioMart (New York, NY, USA), PLGA Resomer RG 502 H Mw 17 kDa was purchased from Boehringer Ingelheim (Biberach, Germany).. Polycaprolactone (PCL) (molecular weight 45 kDa) and PLGA were purchased from Sigma/Aldrich (St. Louis, Missouri, USA).Polycaprolactone (PCL) (molecular weight 45 kDa) and PLGA were purchased from Sigma/Aldrich (St. Louis, Missouri, USA).

Bone Morphogenetic Protein-2 Adsorption onto Poly-?-caprolactone Better Preserves Bioactivity In Vitro and Produces More Bone In Vivo than Conjugation Under Clinically Relevant Loading Scenarios

Journal: Tissue Engineering Part C: Methods    Data: 2015/5/1

Authors: Janki J. Patel, Colleen L. Flanagan, Scott J. Hollister

Article Snippet:The samples were prewashed with activation buffer (BuPH Phosphate-Buffered Saline Pack in diH2O, pH 7.2 Pierce Biotechnology).The samples were prewashed with activation buffer (BuPH Phosphate-Buffered Saline Pack in diH2O, pH 7.2 Pierce Biotechnology).. The heterobifunctional crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane1-carboxylate (sulfo-SMCC; Pierce Biotechnology) was used to immobilize recombinant human BMP2 (Escherichia coli derived; Creative BioMart) on the aminated surface.. Samples were immersed in sulfo-SMCC (4 mg/mL in activation buffer) for 1 h at 23 C, followed by activation and conjugation buffer washes.Samples were immersed in sulfo-SMCC (4 mg/mL in activation buffer) for 1 h at 23 C, followed by activation and conjugation buffer washes.

Cilomilast enhances osteoblast differentiation of mesenchymal stem cells and bone formation induced by bone morphogenetic protein 2.

Journal: Biochimie    PubMed ID: 22706281    Data: 2013/3/5

Authors: Maria Chiara Munisso, Jeong-Hun Kang, Tetsuji Yamaoka

Article Snippet:Osteogenic medium (OM) consisted of standard medium supplemented with 50 mM ascorbate, 1 mM dexamethasone, 2 mM glutamine, and 10 mM b-glycerophosphate.Osteogenic medium (OM) consisted of standard medium supplemented with 50 mM ascorbate, 1 mM dexamethasone, 2 mM glutamine, and 10 mM b-glycerophosphate.. Recombinant human BMP-2 (1 mg/300 mL in acetic acid; Creative BioMart, Shirley, NY, USA) was stored at 20 C. Standard medium containing 30 or 300 ng/mL BMP-2 was freshly prepared.. Cilomilast (Ariflo /SB-207499, S1455; Selleckchem, Houston, TX, USA) and rolipram (R6520; Sigma-Aldrich, Japan) were dissolved in 1 mL of dimethylsulphoxide (DMSO) to obtain 4 mM solutions.Cilomilast (Ariflo /SB-207499, S1455; Selleckchem, Houston, TX, USA) and rolipram (R6520; Sigma-Aldrich, Japan) were dissolved in 1 mL of dimethylsulphoxide (DMSO) to obtain 4 mM solutions.