ActiveRecombinant Human BMPR1B protein, Fc-tagged

• Cat.No.: BMPR1B-8534H
• Product Overview: ActiveRecombinant Human BMPR1B protein(Lys14-Arg126) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 14-126 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit rhBMP4 induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 2-8 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
• Molecular Mass: The recombinant human ALK6/Fc is a disulfide-linked homodimer. The reduced monomer comprises 354 amino acids and has a predicted molecular mass of 39.7 kDa. The apparent molecular mass of the protein is approximately 110 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: (54.4+36.1) % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BMPR1B bone morphogenetic protein receptor, type IB [ Homo sapiens ]
• Official Symbol: BMPR1B
• Synonyms: BMPR1B; bone morphogenetic protein receptor, type IB; bone morphogenetic protein receptor type-1B; ALK6; CDw293; BMPR-1B; BMP type-1B receptor; activin receptor-like kinase 6; serine/threonine receptor kinase; ALK-6
• Gene ID: 658
• mRNA Refseq: NM_001203
• Protein Refseq: NP_001194
• MIM: 603248
• UniProt ID: O00238