Active Recombinant Human BTLA Protein, Mouse IgG2a Fc Tagged

• Cat.No.: BTLA-193H
• Product Overview: Human BTLA (31-150), Mouse IgG2a Fc Tag is expressed from human 293 cells (HEK293). It contains AA Lys 31-Ser 150 (Accession # AAP44003.1). This protein carries a mouse IgG2a Fc tag at the C-terminus. Predicted N-terminus: Lys 31

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: 31-150 aa
• Description: B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA/CD272 inhibits lymphocytes during immune response. BTLA/CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind Human BTLA (31-150), Mouse IgG2a Fc Tag with a linear range of 10-156 ng/mL.
• Molecular Mass: The protein has a calculated MW of 40.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: BTLA B and T lymphocyte associated [ Homo sapiens (human) ]
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743
• Gene ID: 151888
• mRNA Refseq: NM_001085357
• Protein Refseq: NP_001078826
• MIM: 607925
• UniProt ID: Q7Z6A9