Recombinant Human BTLA protein, Fc-tagged

• Cat.No.: BTLA-192H
• Product Overview: Recombinant Human BTLA (Lys31-Thr134) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA/CD272 inhibits lymphocytes during immune response. BTLA/CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
• Source: HEK293
• Species: Human
• Tag: human IgG1 Fc
• Predicted N Terminal: Lys31
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 38.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Lys31-Thr134
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: BTLA
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743
• Gene ID: 151888
• mRNA Refseq: NM_001085357
• Protein Refseq: NP_001078826
• MIM: 607925
• UniProt ID: Q7Z6A9

Reviews

01/25/2023

Very satisfied with a buying experience! Worth it!

04/01/2022

The staff actively communicated with me and solved many technical problems.

03/28/2022

The BTLA proteins purchased from your company is suitable for ELISA.

Q&As

What are the effects of inhibiting BTLA expression?

Inhibition of BTLA expression leads to disruption of autoimmune homeostasis in the organism.

Where is BTLA located?

BTLA is localized in the cell membrane.

What organs are BTLA expressed in?

BTLA is widely expressed in lymphocytes of the bone marrow, thymus and spleen.

What is the biological function of BTLA?

BTLA regulates T and B lymphocyte expression and plays a negative regulatory role in the activation phase of T lymphocytes.