Recombinant Human BTLA protein, Fc-tagged

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Recombinant Human BTLA protein, Fc-tagged

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Cat.No. : BTLA-192H
Product Overview : Recombinant Human BTLA (Lys31-Thr134) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : B- and T-lymphocyte attenuator (BTLA) is also known as B- and T-lymphocyte-associated protein, CD antigen CD272. BTLA contains one Ig-like V-type (immunoglobulin-like) domain. As a lymphocyte inhibitory receptor, BTLA/CD272 inhibits lymphocytes during immune response. BTLA/CD272 can interact with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2, and interact with TNFRSF14/HVEM.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Lys31
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 38.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Lys31-Thr134
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of BTLA-192H :

Human BTLA, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA of BTLA-192H :

Immobilized Human HVEM, His Tag at 5 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag with a linear range of 0.02-1.25 μg/mL (QC tested).

Gene Name : BTLA
Official Symbol : BTLA
Synonyms : BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743
Gene ID : 151888
mRNA Refseq : NM_001085357
Protein Refseq : NP_001078826
MIM : 607925
UniProt ID : Q7Z6A9

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