Recombinant Human BTRC protein, His-tagged

• Cat.No.: BTRC-11H
• Product Overview: Recombinant Human BTRC protein(1-365 aa), fused with His tag, was expressed in E. coli,

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 1-365 aa
• AA Sequence: MDPAEAVLQEKALKFMCSMPRSLWL GCSSLADSMPSLRCLYNPGTGALTA FQNSSEREDCNNGEPPRKIIPEKNS LRQTYNSCARLCLNQETVCLASTAM KTENCVAKTKLANGTSSMIVPKQRK LSASYEKEKELCVKYFEQWSESDQV EFVEHLISQMCHYQHGHINSYLKPM LQRDFITALPARGLDHIAENILSYL DAKSLCAAELVCKEWYRVTSDGMLW KKLIERMVRTDSLWRGLAERRGWGQ YLFKNKPPDGNAPPNSFYRALYPKI IQDIETIESNWRCGRHSLQRIHCRS ETSKGVYCLQYDDQKIVSGLRDNTI KIWDKNTLECKRILTGHTGSVLCLQ YDERVIITGSSDSTV
• Purity: 75%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage.

Gene Information

• Gene Name: BTRC beta-transducin repeat containing E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: BTRC
• Synonyms: BTRC; beta-transducin repeat containing E3 ubiquitin protein ligase; beta transducin repeat containing; F-box/WD repeat-containing protein 1A; beta TrCP1; betaTrCP; bTrCP; bTrCP1; FBXW1A; Fwd1; beta-TrCP1; E3RSIkappaB; F-box and WD-repeat protein 1B; pIkappaBalpha-E3 receptor subunit; F-box and WD repeats protein beta-TrCP; FWD1; FBW1A; FBXW1; BETA-TRCP; MGC4643
• Gene ID: 8945
• mRNA Refseq: NM_001256856
• Protein Refseq: NP_001243785
• MIM: 603482
• UniProt ID: Q9Y297