Active Recombinant Human C2 protein, His-tagged

• Cat.No.: C2-1032H
• Product Overview: Recombinant Human C2 protein(Ala 21 - Leu 752), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 83.0 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala21-Leu752
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: C2 complement component 2 [ Homo sapiens ]
• Official Symbol: C2
• Synonyms: C2; complement component 2; complement C2; C3/C5 convertase; complement component C2; CO2; DKFZp779M0311
• Gene ID: 717
• mRNA Refseq: NM_000063
• Protein Refseq: NP_000054
• MIM: 613927
• UniProt ID: P06681

Reviews

02/02/2023

The C2 protein comes highly recommended for its excellent performance in various research applications.

12/25/2020

It has demonstrated exceptional effectiveness in WB experiments, showcasing its reliability and precision in detecting and quantifying specific targets.

05/08/2019

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

Q&As

Can C2 protein be a target for drug development in autoimmune disorders?

Yes, drugs targeting C2 or the complement system are being explored as potential treatments for autoimmune diseases to regulate the immune response.

Can C2 protein be used as a diagnostic marker for immune system disorders?

Yes, the absence or dysfunction of C2 protein can be indicative of immune system disorders, and its levels can be assessed for diagnostic purposes.

Can C2 protein be used in vaccine development?

Understanding C2's role in immune activation may contribute to vaccine design, potentially enhancing the effectiveness of vaccines by targeting the complement system.

How does C2 protein contribute to autoimmune diseases?

Abnormal activation of the complement system, including C2, is associated with some autoimmune diseases where the immune system mistakenly attacks the body's own cells.

Are there genetic factors influencing C2 protein levels and function?

Yes, genetic variations can affect C2 protein expression and function, leading to variations in immune response among individuals.