Active Recombinant Human C4A protein, His-GST-tagged

Cat.No. : C4A-566H
Product Overview : Active Recombinant Human C4A protein(P0C0L5)(680~756 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.
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Species : Human
Source : E.coli
Tag : GST&His
Protein Length : 680-756 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : Complement Component 4a (C4a) is a component of the complement system, which is a cleavage product of the complement C4 protein. C4a has been implicated in various inflammatory and immune responses. It acts as a chemoattractant, recruiting immune cells such as neutrophils and macrophages to the site of inflammation. Additionally, C4a can stimulate the release of pro-inflammatory cytokines and chemokines, further amplifying the immune response. C4a and C4b are the two subunits of C4, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C4a and recombinant mouse C4b. Briefly, C4a was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to C4b-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-C4a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C4a and recombinant mouse C4b was shown,and this effect was in a dose dependent manner.
Molecular Mass : 38.8kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 90% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name C4A complement component 4A (Rodgers blood group) [ Homo sapiens ]
Official Symbol C4A
Synonyms C4A; complement component 4A (Rodgers blood group); complement component 4A; complement C4-A; C4; C4A2; C4A3; C4A4; C4A6; C4B; C4S; CO4; CPAMD2; RG; acidic C4; C4A anaphylatoxin; Rodgers form of C4; acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; C4AD; MGC164979;
Gene ID 720
mRNA Refseq NM_001252204
Protein Refseq NP_001239133
MIM 120810
UniProt ID P0C0L4

Not For Human Consumption!

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