Recombinant Human C5 protein (R885H), His-tagged
Cat.No. : | C5-9288H |
Product Overview : | Recombinant Human C5 (R885H) protein (Gln19-Cys1676) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293). |
- Specification
- Gene Information
- Related Products
Description : | Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
Source : | HEK293 |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Gln19 |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
Molecular Mass : | The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.9 kDa (β chain) and 114.7 kDa (α chain). The protein migrates as 76 kDa (β chain) and 116 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein length : | Gln19-Cys1676 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | C5 |
Official Symbol : | C5 |
Synonyms : | C5; complement component 5; complement C5; CPAMD4; anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; FLJ17816; FLJ17822; MGC142298 |
Gene ID : | 727 |
mRNA Refseq : | NM_001735 |
Protein Refseq : | NP_001726 |
MIM : | 120900 |
UniProt ID : | P01031 |
Products Types
◆ Recombinant Protein | ||
C5-345H | Recombinant Human C5 Protein, His-tagged | +Inquiry |
C5-0052H | Recombinant Human C5 Protein (G981-Q1306), Tag Free | +Inquiry |
C5-348P | Recombinant Pig C5 Protein, His-tagged | +Inquiry |
C5-347R | Recombinant Rat C5 Protein, His-tagged | +Inquiry |
C5-575H | Recombinant Human C5 Protein (Gln19-Cys1676), His-FLAG-tagged | +Inquiry |
◆ Native Protein | ||
C5-53H | Native Human Complement C5 | +Inquiry |
C5-10540H | Active Native Human C5 | +Inquiry |
◆ Lysates | ||
C5-8020HCL | Recombinant Human C5 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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