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Recombinant Mouse C5 protein, His-tagged

Cat.No. : C5-512M
Product Overview : Recombinant Mouse Complement C5, His Tag is expressed from human 293 cells(HEK293). It contains AA Gln19-Glu1680(Accession #P06684-1).
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Description : Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.5 kDa (βchain) and 114.6 kDa (αchain). The protein migrates as 66 kDa (β chain) and 100 kDa (αchain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln19-Glu1680
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μl. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : Hc
Official Symbol : Hc
Synonyms : Complement C5, C5, CPAMD4
Gene ID : 15139
mRNA Refseq : NM_010406.3
Protein Refseq : NP_034536.3
UniProt ID : P06684

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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