Recombinant Human CA9 Protein, GST-Tagged

• Cat.No.: CA9-0250H
• Product Overview: Human CA9 full-length ORF (NP_001207.1, 1 a.a. - 459 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 76.1 kDa
• AA Sequence: MAPLCPSPWLPLLIPAPAPGLTVQL LLSLLLLMPVHPQRLPRMQEDSPLG GGSSGEDDPLGEEDLPSEEDSPREE DPPGEEDLPGEEDLPGEEDLPEVKP KSEEEGSLKLEDLPTVEAPGDPQEP QNNAHRDKEGDDQSHWRYGGDPPWP RVSPACAGRFQSPVDIRPQLAAFCP ALRPLELLGFQLPPLPELRLRNNGH SVQLTLPPGLEMALGPGREYRALQL HLHWGAAGRPGSEHTVEGHRFPAEI HVVHLSTAFARVDEALGRPGGLAVL AAFLEEGPEENSAYEQLLSRLEEIA EEGSETQVPGLDISALLPSDFSRYF QYEGSLTTPPCAQGVIWTVFNQTVM LSAKQLHTLSDTLWGPGDSRLQLNF RATQPLNGRVIEASFPAGVDSSPRA AEPVQLNSCLAAGDILALVFGLLFA VTSVAFLVQMRRQHRRGTKGGVSYR PAEVAETGA
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CA9 carbonic anhydrase IX [ Homo sapiens ]
• Official Symbol: CA9
• Synonyms: CA9; carbonic anhydrase IX; carbonic anhydrase 9; CAIX; carbonic dehydratase; MN; RCC associated protein G250; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250
• Gene ID: 768
• mRNA Refseq: NM_001216
• Protein Refseq: NP_001207
• MIM: 603179
• UniProt ID: Q16790

Reviews

08/04/2022

Exceptional consistency, vital for our research's reliability.

12/16/2021

Critical support for our protein analysis and quantification.

02/07/2021

Unmatched accuracy in protein identification and sequencing.

Q&As

What signaling pathways are known to regulate CA9 expression?

The HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.

How does CA9 contribute to pH regulation within the tumor microenvironment?

CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.

How is CA9 expression regulated in hypoxic conditions?

CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.

How are elevated levels of CA9 associated with cancer prognosis?

Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.

What post-translational modifications have been identified on CA9?

Potential modifications like glycosylation can be identified using techniques like mass spectrometry.

Are there specific inhibitors that target CA9, especially in cancer therapeutics?

Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.

What roles does CA9 play in tumor physiology?

CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.