Recombinant Human CA9 protein, His-Avi-tagged

• Cat.No.: CA9-790H
• Product Overview: Recombinant Human Carbonic Anhydrase IX/CA9 Protein is expressed from mammalian with a His Tag and Avi Tag at the C-terminal. It contains Gln 38-Asp 414.

Specification

• Description: CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
• Source: HEK293
• Species: Human
• Tag: His&Avi
• Form: Supplied as 0.22um filtered solution in 50mM Tris,150mM NaCl PH 7.5.
• Molecular Mass: The protein has a predicted MW of 43.7 kDa.Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result.
• Protein length: Gln 38-Asp 414
• Endotoxin: Less than 0.05 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles

Gene Information

• Gene Name: CA9 carbonic anhydrase 9 [Homo sapiens (human)]
• Official Symbol: CA9
• Synonyms: CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250
• Gene ID: 768
• mRNA Refseq: NM_001216.3
• Protein Refseq: NP_001207.2
• MIM: 603179
• UniProt ID: Q16790

Reviews

08/04/2022

Exceptional consistency, vital for our research's reliability.

12/16/2021

Critical support for our protein analysis and quantification.

02/07/2021

Unmatched accuracy in protein identification and sequencing.

Q&As

What signaling pathways are known to regulate CA9 expression?

The HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.

How does CA9 contribute to pH regulation within the tumor microenvironment?

CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.

How is CA9 expression regulated in hypoxic conditions?

CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.

How are elevated levels of CA9 associated with cancer prognosis?

Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.

What post-translational modifications have been identified on CA9?

Potential modifications like glycosylation can be identified using techniques like mass spectrometry.

Are there specific inhibitors that target CA9, especially in cancer therapeutics?

Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.

What roles does CA9 play in tumor physiology?

CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.