Recombinant Human CACNA1D Protein, His&GST-tagged

• Cat.No.: CACNA1D-352H
• Product Overview: Recombinant Human CACNA1D Protein (Met1-Lys163) with N-His&GST tag was expressed in E. coli.

Specification

• Description: Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 48.1 kDa
  Accurate Molecular Mass: 48 kDa
• Protein length: Met1-Lys163
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: CACNA1D calcium channel, voltage-dependent, L type, alpha 1D subunit [ Homo sapiens (human) ]
• Official Symbol: CACNA1D
• Synonyms: CACH3; CACN4; Cav1.3; CCHL1A2; CACNL1A2
• Gene ID: 776
• mRNA Refseq: NM_001128840
• Protein Refseq: NP_001122312
• MIM: 114206
• UniProt ID: Q01668