Recombinant Human CAPN2 Protein, GST-Tagged

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Recombinant Human CAPN2 Protein, GST-Tagged

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Cat.No. : CAPN2-0363H
Product Overview : Human CAPN2 full-length ORF (AAH21303.1, 1 a.a. - 700 a.a.) recombinant protein with GST-tag at N-terminal.
Description : The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 106.4 kDa
AA Sequence : MAGIAAKLAK DREAAEGLGS HERAIKYLNQ DYEALRNECL EAGTLFQDPS FPAIPSALGF KELGPYSSKT RGIEWKRPTE ICADPQFIIG GATRTDICQG ALGDCWLLAA IASLTLNEEI LARVVPLNQS FQENYAGIFH FQFWQYGEWV EVVVDDRLPT KDGELLFVHS AEGSEFWSAL LEKAYAKING CYEALSGGAT TEGFEDFTGG IAEWYELKKP PPNLFKIIQK ALQKGSLLGC SIDITSAADS EAITFQKLVK GHAYSVTGAE EVESNGSLQK LIRIRNPWGE VEWTGRWNDN CPSWNTIDPE ERERLTRRHE DGEFWMSFSD FLRHYSRLEI CNLTPDTLTS DTYKKWKLTK MDGNWRRGST AGGCRNYPNT FWMNPQYLIK LEEEDEDEED GESGCTFLVG LIQKHRRRQR KMGEDMHTIG FGIYEVPEEL SGQTNIHLSK NFFLTNRARE RSDTFINLRE VLNRFKLPPG EYILVPSTFE PNKDGDFCIR VFSEKKADYQ AVDDEIEANL EEFDISEDDI DDGFRRLFAQ LAGEDAEISA FELQTILRRV LAKRQDIKSD GFSIETCKIM VDMLDSDGSG KLGLKEFYIL WTKIQKYQKI YREIDVDRSG TMNSYEMRKA LEEAGFKMPC QLHQVIVARF ADDQLIIDFD NFVRCLVRLE TLFKIFKQLD PENTGTIELD LISWLCFSVL
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Quality Control Testing :

12.5% SDS-PAGE Stained with Coomassie Blue.

Gene Name : CAPN2 calpain 2, (m/II) large subunit [ Homo sapiens ]
Official Symbol : CAPN2
Synonyms : CAPN2; calpain 2, (m/II) large subunit; calpain-2 catalytic subunit; CANPL2; CANPml; mCANP; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neutral proteinase 2; CANP2; FLJ39928;
Gene ID : 824
mRNA Refseq : NM_001146068
Protein Refseq : NP_001139540
MIM : 114230
UniProt ID : P17655

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