Active Native Human Cathepsin H

• Cat.No.: CTSH-1650H
• Product Overview: Active Native Human CTSH Protein purified from human liver. Extinction Coefficient (E) = 1.22 (0.1% at 280 nm, 1 cm pathway)

Specification

• Species: Human
• Source: Liver
• Description: Cathepsin H (CatH) is a lysosomal cysteine protease distinguished by its unique dual aminopeptidase and endopeptidase activities. Unlike other cathepsins, it includes a disulfide-linked mini-chain that anchors substrate N-termini via electrostatic interactions, enabling sequential cleavage of N-terminal basic residues (Arg/Lys). This exopeptidase function, optimal at neutral pH, facilitates its role in extracellular spaces, while its endopeptidase activity at acidic pH (4.5–5.5) supports lysosomal protein degradation.
• Form: Frozen in 20 mM sodium acetate, pH 4.5, with 1 mM EDTA and 100 mM NaCl
• Bio-activity: > 1 unit/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of L-arginine-beta-napthylamide per minute at 40 centigrade using 75 mM potassium phosphate, pH 6.8, with 1 mM EDTA and 3 mM cysteine as the activation buffer.
• Molecular Mass: 28 kDa
• Purity: ≥ 90% (SDS-PAGE)
• Storage: ≤ -70 centigrade

Gene Information

• Gene Name: CTSH cathepsin H [ Homo sapiens (human) ]
• Official Symbol: CTSH
• Synonyms: CTSH; cathepsin H; ACC4; ACC5; CPSB; ACC-4; ACC-5; pro-cathepsin H; N-benzoylarginine-beta-naphthylamide hydrolase; aleurain; cathepsin B3; cathepsin BA; EC 3.4.22.16
• Gene ID: 1512
• mRNA Refseq: NP_004381
• Protein Refseq: NM_004390
• MIM: 116820
• UniProt ID: P09668