Recombinant Human CCL7 protein

• Cat.No.: CCL7-286H
• Product Overview: Recombinant Human CCL7 protein was expressed in Escherichia coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 76
• Description: Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Bio-activity: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
• Molecular Mass: Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids.
• AA Sequence: QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWVQDFMKHLDKKTQTPKL
• Endotoxin: Less than 1 EU/μg of rHuMCP-3/CCL7 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: CCL7
• Official Symbol: CCL7
• Synonyms: CCL7; chemokine (C-C motif) ligand 7; SCYA6, SCYA7, small inducible cytokine A7 (monocyte chemotactic protein 3); C-C motif chemokine 7; FIC; MARC; MCP 3; MCP3; monocyte chemoattractant protein 3; monocyte chemotactic protein 3; NC28; small-inducible cytokine A7; small inducible cytokine A7 (monocyte chemotactic protein 3); MCP-3; SCYA6; SCYA7; MGC138463; MGC138465
• Gene ID: 6354
• mRNA Refseq: NM_006273
• Protein Refseq: NP_006264
• MIM: 158106
• UniProt ID: P80098