CCL7-286H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant Human CCL7 protein was expressed in Escherichia coli.
Description :
Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells.
Source :
E.coli
Species :
Human
Form :
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Bio-activity :
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Molecular Mass :
Approximately 9.0 kDa, a single, non-glycosylated polypeptide chain containing 76 amino acids.
Less than 1 EU/μg of rHuMCP-3/CCL7 as determined by LAL method.
Purity :
>97% by SDS-PAGE and HPLC analysis.
Storage :
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution :
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.