Active Recombinant Human CCNE1 Protein, His & GST-tagged

• Cat.No.: CCNE1-259H
• Product Overview: Recombinant full length of human CCNE1 isoform 1 (NP_001229.1) (Met 1-Ala 410) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His & GST
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, 1mM GSH, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human HG3C-CCNE1 at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml.
• Molecular Mass: The recombinant human CCNE1/GST chimera consists of 647 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >82 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CCNE1 cyclin E1 [ Homo sapiens ]
• Official Symbol: CCNE1
• Synonyms: CCNE1; cyclin E1; CCNE; G1/S-specific cyclin-E1; cyclin Es; cyclin Et
• Gene ID: 898
• mRNA Refseq: NM_001238
• Protein Refseq: NP_001229
• MIM: 123837
• UniProt ID: P24864
• Chromosome Location: 19q12
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem