Recombinant Human CD14 Protein, C-His-tagged

• Cat.No.: CD14-179H
• Product Overview: Recombinant Human CD14 Protein with C-His tag was expressed in E. coli.

Specification

• Description: CD14 is a leucine-rich repeat-containing pattern recognition receptor with expression largely restricted to the monocyte/macrophage cell lineage. Research studies have shown that CD14 is a bacterial lipopolysaccharide (LPS) binding glycoprotein, expressed as either a GPI-linked membrane protein or a soluble plasma protein. LPS induces an upregulation of GPI-linked CD14 expression, which facilitates TLR4 signaling and macrophage activation in response to bacterial infection. The 61D3 antibody is widely used to identify cells of the monocyte/macrophage lineage.
• Source: E. coli
• Species: Human
• Tag: C-His
• Molecular Mass: ~36 kDa
• AA Sequence: TTPEPCELDDEDFRCVCNFSEPQPD WSEAFQCVSAVEVEIHAGGLNLEPF LKRVDADADPRQYADTVKALRVRRL TVGAAQVPAQLLVGALRVLAYSRLK ELTLEDLKITGTMPPLPLEATGLAL SSLRLRNVSWATGRSWLAELQQWLK PGLKVLSIAQAHSPAFSCEQVRAFP ALTSLDLSDNPGLGERGLMAALCPH KFPAIQNLALRNTGMETPTGVCAAL AAAGVQPHSLDLSHNSLRATVNPSA PRCMWSSALNSLNLSFAGLEQVPKG LPAKLRVLDLSCNRLNRAPQPDELP EVDNLTLDGNPFLVPGTALPHEGSM N
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Concentration: ≥0.5 mg/mL
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: CD14 CD14 molecule [ Homo sapiens (human) ]
• Official Symbol: CD14
• Synonyms: CD14; CD14 molecule; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
• Gene ID: 929
• mRNA Refseq: NM_000591
• Protein Refseq: NP_000582
• MIM: 158120
• UniProt ID: P08571