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Recombinant Human CD19 Protein, LIgG2b Fc-tagged, low endotoxin

Cat.No. : CD19-340H
Product Overview : Recombinant Human CD19 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1). Predicted N-terminus: Pro 20. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 57.9 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Human
Tag : LIgG2b Fc
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized FMC63 at 0.5 μg/mL (100 μL/well) can bind Recombinant Human CD19 Protein, LIgG2b Fc-tagged, low endotoxin with a linear range of 0.078-1.25 μg/mL (QC tested).
Molecular Mass : 57.9 kDa
Protein length : Pro 20 - Lys 291
Endotoxin : Less than 0.01 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : CD19 CD19 molecule [ Homo sapiens ]
Official Symbol : CD19
Synonyms : CD19; CD19 molecule; CD19 antigen; B4; CVID3; MGC12802;
Gene ID : 930
mRNA Refseq : NM_001178098
Protein Refseq : NP_001171569
MIM : 107265
UniProt ID : P15391

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