Active Recombinant Human CD19 Protein, LIgG2b Fc-tagged, low endotoxin

• Cat.No.: CD19-340H
• Product Overview: Recombinant Human CD19 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1). Predicted N-terminus: Pro 20. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 57.9 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: LIgG2b Fc
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized FMC63 at 0.5 μg/mL (100 μL/well) can bind Recombinant Human CD19 Protein, LIgG2b Fc-tagged, low endotoxin with a linear range of 0.078-1.25 μg/mL (QC tested).
• Molecular Mass: 57.9 kDa
• Protein length: Pro 20 - Lys 291
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD19 CD19 molecule [ Homo sapiens ]
• Official Symbol: CD19
• Synonyms: CD19; CD19 molecule; CD19 antigen; B4; CVID3; MGC12802
• Gene ID: 930
• mRNA Refseq: NM_001178098
• Protein Refseq: NP_001171569
• MIM: 107265
• UniProt ID: P15391