|Product Overview :||Recombinant Human CD27 Protein (Met1-Ile192) (P26842) was produced by Baculovirus-Insect Cells expression system. This protein was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.|
|Description :||CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD7 on lymphocytes and dendritic cells. The CD27-CD7 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.|
|Source :||Baculovirus-Insect Cells|
|Predicted N Terminal :||Ala 20|
|Form :||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.|
|Bio-activity :||1.Measured by its binding ability in a functional ELISA.
2.Immobilized biotinylated human CD27-His at 10μg/mL (100μL/well) can bind human CD70-Fc, The EC50 of biotinylated human CD70-Fc is 8-25ng/mL.
|Molecular Mass :||The secreted recombinant human CD27 consists of 183 amino acids and predicts a molecular mass of 20.7 KDa.|
|Protein length :||Met1-Ile192|
|Endotoxin :||< 1.0 EU per μg protein as determined by the LAL method.|
|Purity :||> 90 % as determined by SDS-PAGE|
|Stability :||Samples are stable for up to twelve months from date of receipt at -70 centigrade.|
|Storage :||Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|Shipping :||In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|Gene Name :||CD27 CD27 molecule [ Homo sapiens ]|
|Official Symbol :||CD27|
|Synonyms :||CD27; CD27 molecule; CD27 antigen; S152; Tp55; CD27L receptor; T14; TNFRSF7; MGC20393;|
|Gene ID :||939|
|mRNA Refseq :||NM_001242|
|Protein Refseq :||NP_001233|
|UniProt ID :||P26842|