Recombinant Human CD274 protein, Avi & His-tagged, Biotinylated

Cat.No.: CD274-1283H
Product Overview: Biotinylated Human PD-L1 (recommended for biopanning) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Description: Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
Source: HEK293
Species: Human
Tag : Avi & His
Predicted N Terminal: Phe 19
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass: The protein has a calculated MW of 27.8 kDa. The protein migrates as 38-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length: Phe 19 - Arg 238
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: CD274 CD274 molecule [ Homo sapiens (human) ]
Official Symbol: CD274
Synonyms: B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed cell death 1 ligand 1
Gene ID: 29126
mRNA Refseq: NM_001267706.1
Protein Refseq: NP_001254635.1
UniProt ID: Q9NZQ7

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