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Active Recombinant Human CD274 protein, Avi-His-tagged, Biotinylated

Cat.No. : CD274-1283H
Product Overview : Biotinylated Recombinant Human CD274 protein(Phe 19 - Arg 238), fused with Avi and His tag, was expressed in HEK293.
  • Specification
  • Gene Information
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Source : HEK293
Species : Human
Tag : Avi-His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Bio-activity : Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified) with a linear range of 0.1-2 ng/mL. Immobilized Biotinylated Human PD-L1, Avitag,His Tag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Anti-PD-L1 MAb, Human IgG1 with a linear range of 0.2-3 ng/mL.
Molecular Mass : This protein carries an Avi tag (Avitag) at the C-terminus, followed by a polyhistidine tagThe protein has a calculated MW of 27.8 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Phe 19 - Arg 238
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : CD274 CD274 molecule [ Homo sapiens ]
Official Symbol : CD274
Synonyms : CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;
Gene ID : 29126
mRNA Refseq : NM_014143
Protein Refseq : NP_054862
MIM : 605402
UniProt ID : Q9NZQ7

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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