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Recombinant Human CD28 Protein

Cat.No.: CD28-321H
Product Overview: Recombinant human CD28 (P10747-1) (Met 1-Pro 152) was expressed with six amino acids (LEVLFQ) at the C-terminus.
Source: HEK293
Species: Human
Predicted N Terminal: Asn 19
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant human CD28 consists of 141 amino acids and predicts a molecular mass of 15.9 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: CD28 CD28 molecule [ Homo sapiens ]
Official Symbol: CD28
Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290;
Gene ID: 940
mRNA Refseq: NM_001243077
Protein Refseq: NP_001230006
MIM: 186760
UniProt ID: P10747
Chromosome Location: 2q33
Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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