Recombinant Human CD33 Protein, His-tagged

• Cat.No.: CD33-190H
• Product Overview: Recombinant Human CD33 Protein with His tag was expressed in E. coli.

Specification

• Description: CD33, a type I transmembrane protein, is a sialic acid-binding Ig-like lectin (Siglec-3) of the Ig superfamily, and human CD33 binds preferentially to alpha-2, 6-linked sialic acid. Upon binding to its ligands CD33 transduces an inhibitory signaling through the immunoreceptor tyrosine-based inhibitory motif (ITIM) in its intracellular domain, inhibiting cellular function such as phagocytosis. In addition, CD33 is also involved in other processes, such as adhesion. Due to its exclusive expression on hematopoietic cells, particularly the myeloid lineage and their progenitors, CD33 has been actively pursued as a therapeutic target against acute myeloid leukemia (AML). CD33 may also be involved in Alzheimer’s Disease.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~33 kDa
• AA Sequence: DPNFWLQVQESVTVQEGLCVLVPCT FFHPIPYYDKNSPVHGYWFREGAII SRDSPVATNKLDQEVQEETQGRFRL LGDPSRNNCSLSIVDARRRDNGSYF FRMERGSTKYSYKSPQLSVHVTDLT HRPKILIPGTLEPGHSKNLTCSVSW ACEQGTPPIFSWLSAAPTSLGPRTT HSSVLIITPRPQDHGTNLTCQVKFA GAGVTTERTIQLNVTYVPQNPTTGI FPGDGSGKQETRAGVVH
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: CD33 CD33 molecule [ Homo sapiens (human) ]
• Official Symbol: CD33
• Synonyms: CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3
• Gene ID: 945
• mRNA Refseq: NM_001082618
• Protein Refseq: NP_001076087
• MIM: 159590
• UniProt ID: P20138

Reviews

11/14/2019

Quick dissolution, easy use.

08/27/2019

Excellent solubility, no aggregates.

02/27/2019

High concentration, very effective.

Q&As

What are the implications of CD33 polymorphisms in therapeutic targeting?

Certain polymorphisms in the CD33 gene can influence its expression on the cell surface, which might affect the efficacy of CD33-targeted therapies.

What is the primary biological function of CD33 in hematopoietic cells?

CD33 is a transmembrane receptor primarily expressed on cells of myeloid lineage and is involved in modulating cellular functions, including the regulation of cellular proliferation and differentiation.

How does CD33 expression vary among different leukocyte populations?

CD33 is predominantly expressed on myeloid cells, especially on immature myeloid cells. Its expression decreases as these cells differentiate and is typically low or absent on lymphoid cells.

How has CD33 been utilized as a therapeutic target in hematological malignancies?

CD33 has been targeted for therapeutic antibodies in AML. An example is gemtuzumab ozogamicin, which targets CD33 and delivers a cytotoxic agent specifically to leukemia cells expressing CD33.

How does CD33 signaling influence myeloid cell differentiation?

While the exact signaling pathways of CD33 are still under investigation, it is believed that CD33 signaling can influence the differentiation and function of myeloid cells, potentially playing a role in immune regulation.

What role does CD33 play in acute myeloid leukemia (AML)?

CD33 is commonly expressed on the surface of AML blasts, making it a potential target for therapeutic interventions in AML.

Are there known ligands that bind specifically to CD33?

The specific ligands for CD33 are not fully characterized, but it is believed to play a role in cell-cell interactions within the immune system.