Active Recombinant Human CD40 Protein, Fc-His-tagged

• Cat.No.: CD40-31H
• Product Overview: Active Recombinant Human CD40 Protein(NP_001241.1)(Met 1-Arg193) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-193 aa
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD40 at 2 μg/ml (100 μl/well) can bind biotinylated human CD40L with a linear range of 7.8-125 ng/ml.
• Molecular Mass: The recombinant human CD40/Fc is a disulfide-linked homodimer. The reduced monomer consists of 421 amino acids and has a predicted molecular mass of 47.3 kDa. As a result of glycosylation, the apparent molecular mass of rhCD40/Fc monomer migrates with an apparent molecular mass of 55-60 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ]
• Official Symbol: CD40
• Synonyms: CD40; CD40 molecule, TNF receptor superfamily member 5; TNFRSF5, tumor necrosis factor receptor superfamily, member 5; tumor necrosis factor receptor superfamily member 5; Bp50; p50; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily, member 5; nerve growth factor receptor-related B-lymphocyte activation molecule; CDW40; TNFRSF5; MGC9013
• Gene ID: 958
• mRNA Refseq: NM_001250
• Protein Refseq: NP_001241
• MIM: 109535
• UniProt ID: P25942