Active Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated
Cat.No. : | CD40-371H |
Product Overview : | Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1). Predicted N-terminus: Glu 21. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. The protein has a calculated MW of 48.1 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc & Avi |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 0.313-2.5 μg/mL (QC tested). FACS analysis shows that Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated can bind to 293T cells overexpressing human CD40L. The concentration of Biotinylated Human CD40 is 0.1 μg/ml (Routinely tested). |
Molecular Mass : | 48.1 kDa |
Protein length : | Glu 21 - Arg 193 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ] |
Official Symbol : | CD40 |
Synonyms : | CD40; CD40 molecule, TNF receptor superfamily member 5; Bp50; p50; CD40L receptor; CDW40; TNFRSF5; MGC9013; |
Gene ID : | 958 |
mRNA Refseq : | NM_001250 |
Protein Refseq : | NP_001241 |
MIM : | 109535 |
UniProt ID : | P25942 |
Products Types
◆ Recombinant Protein | ||
Cd40-1462M | Recombinant Mouse Cd40 Protein, His (Fc)-Avi-tagged | +Inquiry |
CD40-32H | Recombinant Human CD40 Protein (ECD), His-tagged(C-ter) | +Inquiry |
Cd40-8727R | Recombinant Rat Cd40 protein(Met1-Arg193), His-tagged | +Inquiry |
CD40-816M | Recombinant Mouse CD40 Protein (Val24-Arg193), His-tagged | +Inquiry |
CD40-633R | Recombinant Rhesus macaque CD40 Protein, Fc-tagged | +Inquiry |
◆ Lysates | ||
CD40-1254CCL | Recombinant Cynomolgus CD40 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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