Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated

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Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated

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Cat.No. : CD40-371H
Product Overview : Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1). Predicted N-terminus: Glu 21. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag. The protein has a calculated MW of 48.1 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Source : HEK293
Species : Human
Tag : Fc & Avi
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human CD40 Ligand, His Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated with a linear range of 0.313-2.5 μg/mL (QC tested).
FACS analysis shows that Recombinant Human CD40 Protein, Fc & Avi-tagged, Biotinylated can bind to 293T cells overexpressing human CD40L. The concentration of Biotinylated Human CD40 is 0.1 μg/ml (Routinely tested).
Molecular Mass : 48.1 kDa
Protein length : Glu 21 - Arg 193
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : CD40 CD40 molecule, TNF receptor superfamily member 5 [ Homo sapiens ]
Official Symbol : CD40
Synonyms : CD40; CD40 molecule, TNF receptor superfamily member 5; Bp50; p50; CD40L receptor; CDW40; TNFRSF5; MGC9013;
Gene ID : 958
mRNA Refseq : NM_001250
Protein Refseq : NP_001241
MIM : 109535
UniProt ID : P25942

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