Active Recombinant Human CD40LG protein, Fc-tagged

• Cat.No.: CD40LG-279H
• Product Overview: Active Recombinant Human CD40LG protein(NP_000065.1) (Met 113-Leu 261) was expressed in HEK293, fused with the Fc region of human IgG1 at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 113-261 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human CD40 at 2 μg/ml (100 μl/well) can bind human CD40L Fc Chimera with a linear ranger of 1.28-32 ng/ml.
• Molecular Mass: The recombinant human CD40L/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 386 amino acids and has a predicted molecular mass of 42.8 kDa. The apparent molecular mass of rh CD40L/Fc monomer is approximately 45 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD40LG CD40 ligand [ Homo sapiens ]
• Official Symbol: CD40LG
• Synonyms: CD40LG; CD40 ligand; HIGM1, IMD3, TNFSF5, tumor necrosis factor (ligand) superfamily, member 5 (hyper IgM syndrome); CD40 antigen ligand; CD40L; CD154; gp39; hCD40L; hyper IgM syndrome; T B cell activating molecule; TNF related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; CD40-L; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; IGM; IMD3; HIGM1; T-BAM; TNFSF5
• Gene ID: 959
• mRNA Refseq: NM_000074
• Protein Refseq: NP_000065
• MIM: 300386
• UniProt ID: P29965