Active Recombinant Human CD46 Protein, His Tagged

• Cat.No.: CD46-3962H
• Product Overview: Human CD46, His Tag is expressed from human 293 cells (HEK293). It contains AA Cys 35-Asp 328 (Accession # P15529-3). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Cys 35

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 35-328 aa
• Description: Complement regulatory protein CD46 is also known as membrane Cofactor Protein (MCP), is a type I membrane protein and is a regulatory part of the complement system. CD46 is expressed by all cells except erythrocytes. MCP acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue, and also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. In T-cells by binding to CD46, A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2).
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human CD46, His Tag at 1 μg/mL (100 μL/well) can bind FG-3246 (FOR46), Human IgG1 kappa with a linear range of 0.3-2 ng/mL.
• Molecular Mass: The protein has a calculated MW of 33.6 kDa. The protein migrates as 45-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD46 CD46 molecule, complement regulatory protein [ Homo sapiens (human) ]
• Official Symbol: CD46
• Synonyms: CD46; CD46 molecule, complement regulatory protein; antigen identified by monoclonal TRA 2 10, CD46 antigen, complement regulatory protein, MCP, membrane cofactor protein (CD46, trophoblast lymphocyte cross reactive antigen), MIC10; membrane cofactor protein; MGC26544; TLX; TRA2.10; measles virus receptor; complement membrane cofactor protein; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; CD46 antigen, complement regulatory protein; trophoblast-lymphocyte cross-reactive antigen; antigen identified by monoclonal TRA-2-10; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MCP; AHUS2; MIC10
• Gene ID: 4179
• mRNA Refseq: NM_002389
• Protein Refseq: NP_002380
• MIM: 120920
• UniProt ID: P15529