Recombinant Human CD47 protein, Fc-tagged (HPLC-verified)

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Recombinant Human CD47 protein, Fc-tagged (HPLC-verified)

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Cat.No. : CD47-527H
Product Overview : Recombinant Human CD47 (Gln19-Pro139) protein (HPLC-verified) was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Gln19
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 40.4 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln19-Pro139
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD47-527H :

Human CD47, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC of CD47-527H :

The purity of Human CD47, Fc Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA :

Immobilized Human CD47, Fc Tag (HPLC-verified) at 2 μg/mL (100 μL/well) can bind Human SIRP alpha, His Tag (HPLC-verified) with a linear range of 0.016-0.5 μg/mL (QC tested).

Bioactivity-ELISA of CD47-527H :

Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag (HPLC-verified) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 μg/mL (Routinely tested).

Bioactivity-BLI of CD47-527H :

Immobilized Human CD47, Fc Tag (HPLC-verified) on AHC Biosensor, can bind Human SIRP alpha, His Tag (HPLC-verified) with an affinity constant of 0.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS of CD47-527H :

FACS assay shows that Human CD47, Fc Tag (HPLC-verified) can bind to ACHN cell expressing SIRP-a. The concentration of CD47 used is 10 μg/mL (Routinely tested).

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 10 μg/mL. IC50=15.02 μg/mL (Routinely tested).

Gene Name : CD47
Official Symbol : CD47
Synonyms : CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer), MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6
Gene ID : 961
mRNA Refseq : NM_001777
Protein Refseq : NP_001768
MIM : 601028
UniProt ID : Q08722

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