Active Recombinant Human CD47 protein, Fc/Avi-tagged, Biotinylated

• Cat.No.: CD47-7890H
• Product Overview: Biotinylated Recombinant Human CD47 (Gln 19 - Pro 139) fused with human IgG1 Fc fragment at the C-terminus, followed by an Avi tag was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc/Avi, Biotinylated
• Predicted N Terminal: Gln 19
• Form: Lyophilized from 0.22µm filtered solution in 50 mM tris, 100 mM glycine (pH7.5) with 10% Trehalose as protectant.
• Bio-activity: Immobilized Human SIRP alpha Protein, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47 Protein, Fc Tag with a linear range of 8-250 ng/mL.
• Molecular Mass: The protein has a calculated MW of 42.2 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing condition due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Protein length: Gln 19 - Pro 139
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigradeor lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigradefor 12 months in lyophilized state;-70 centigradefor 3 months under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Gene Information

• Gene Name: CD47 CD47 molecule [ Homo sapiens ]
• Official Symbol: CD47
• Synonyms: CD47; CD47 molecule; CD47 antigen (Rh related antigen, integrin associated signal transducer) , MER6; leukocyte surface antigen CD47; antigen identified by monoclonal 1D8; antigenic surface determinant protein OA3; CD47 glycoprotein; IAP; integrin associated protein; OA3; Rh related antigen; Rh-related antigen; integrin-associated protein; integrin-associated signal transducer; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); MER6
• Gene ID: 961
• mRNA Refseq: NM_198793
• Protein Refseq: NP_942088
• MIM: 601028
• UniProt ID: Q08722
• Chromosome Location: 3q13.1-q13.2
• Pathway: Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hemostasis, organism-specific biosystem; Integrins in angiogenesis, organism-specific biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem
• Function: protein binding; thrombospondin receptor activity