Recombinant Human CD55 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD55-64HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human CD55 precursor (NP_000565.1) (Met 1-Ser 353), fused with the C-terminal Fc region of human IgG1, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: The recombinant human CD55/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 557 amino acids and has a predicted molecular mass of 61.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD55/Fc monomer is approximately 95-105 kDa due to glycosylation.
• Protein length: 557
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: CD55 CD55 molecule, decay accelerating factor for complement (Cromer blood group) [ Homo sapiens ]
• Official Symbol: CD55
• Gene ID: 1604
• mRNA Refseq: NM_000574
• Protein Refseq: NP_000565
• MIM: 125240
• UniProt ID: P08174

Reviews

02/23/2022

Efficient shipping and exceptional product quality. This company knows how to serve its customers.

09/20/2019

Consistency is key in research, and this product delivers that every time.

03/02/2018

We've tried various proteins, but this one stands out for its purity and performance.

Q&As

How do genetic variations in CD55 influence susceptibility to complement-mediated disorders, and what are the molecular mechanisms involved?

Genetic variations in CD55 may influence susceptibility to complement-mediated disorders by affecting the protein's ability to regulate complement activation. This can lead to conditions like paroxysmal nocturnal hemoglobinuria (PNH).

How does CD55 contribute to the modulation of immune responses beyond its role in complement regulation?

CD55 has immunomodulatory functions beyond complement regulation, influencing T cell responses and contributing to the maintenance of immune tolerance.

What role does CD55 play in protecting cells from complement-mediated lysis, and how does it contribute to immune evasion?

CD55 protects cells from complement-mediated lysis by preventing the formation of membrane attack complexes. This contributes to immune evasion by pathogens and self-cells alike.

Can you elaborate on the structural domains of CD55 and how they mediate its interactions with complement components?

CD55 consists of extracellular domains responsible for interactions with complement proteins, particularly C3b and C4b. These interactions prevent the formation of membrane attack complexes on host cells.

Can you discuss the involvement of CD55 in various diseases, including autoimmune disorders and infectious diseases?

CD55 dysregulation is associated with autoimmune disorders, where it may contribute to immune system overactivity. Additionally, some pathogens exploit CD55 to evade host immune responses.