Recombinant Human CD70 Protein, MIgG2a Fc-tagged, low endotoxin

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Recombinant Human CD70 Protein, MIgG2a Fc-tagged, low endotoxin

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Cat.No. : CD70-328H
Product Overview : Recombinant Human CD70 Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Gln 39 - Pro 193 (Accession # P32970-1). Predicted N-terminus: Glu. This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 44.0 kDa. The protein migrates as 50-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Human
Tag : MIgG2a Fc
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human CD27 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD70 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.2-6 ng/mL (QC tested).
Molecular Mass : 44.0 kDa
Protein length : Gln 39 - Pro 193
Endotoxin : Less than 0.1 EU per μg by the LAL method.
Purity : >85% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : CD70 CD70 molecule [ Homo sapiens ]
Official Symbol : CD70
Synonyms : CD70; CD70 molecule; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; CD27LG; TNFSF7;
Gene ID : 970
mRNA Refseq : NM_001252
Protein Refseq : NP_001243
MIM : 602840
UniProt ID : P32970

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