Active Recombinant Human CD74 Protein, HA-tagged

• Cat.No.: CD74-546H
• Product Overview: Recombinant Human CD74 (Accession # NP_004346) Gln73-Met232, fused with an N-terminal HA (YPYDVPDYA) tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Human
• Source: CHO
• Tag: HA
• Protein Length: Gln73-Met232
• Predicted N Terminal: Tyr
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.When 5 x 10^4 cells per well are added to Recombinant Human CD74 coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is typically 0.6 - 3 µg/mL.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human CD74, HA-tagged has a calculated MW of 19.3 kDa. In SDS-PAGE migrates as 30-35 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in 4 mM HCl containing 0.1% bovine serum albumin.

Gene Information

• Gene Name: CD74 CD74 molecule, major histocompatibility complex, class II invariant chain [ Homo sapiens ]
• Official Symbol: CD74
• Synonyms: CD74; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen associated) , DHLAG; HLA class II histocompatibility antigen gamma chain; gamma chain of class II antigens; HLA DR gamma; Ia associated invariant chain; MHC HLA DR gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); II; DHLAG; HLADG; Ia-GAMMA; FLJ98970
• Gene ID: 972
• mRNA Refseq: NM_001025158
• Protein Refseq: NP_001020329
• MIM: 142790
• UniProt ID: P04233
• Chromosome Location: 5q31-q33