Active Recombinant Human CDH3 protein, His-tagged

• Cat.No.: CDH3-169H
• Product Overview: Recombinant Human CDH3 protein(Asp108-Gly654), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 108-654 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1×10^5 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 50-60% will adhere after 1.5 hours at 37° C.
• Molecular Mass: 60.86 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 85 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: CDH3 cadherin 3, type 1, P-cadherin (placental) [ Homo sapiens ]
• Official Symbol: CDH3
• Synonyms: CDH3; cadherin 3, type 1, P-cadherin (placental); cadherin 3, P cadherin (placental); cadherin-3; CDHP; PCAD; calcium-dependent adhesion protein, placental; HJMD
• Gene ID: 1001
• mRNA Refseq: NM_001793
• Protein Refseq: NP_001784
• MIM: 114021
• UniProt ID: P22223